Hogeschool van Amsterdam

Forensisch onderzoek

Non-marking collection of amino acids from fingerprints using hydrogels


The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualization at a later stage using the standard method of cyanoacrylate fuming followed by basic-yellow dyeing.

Reference van Helmond, W., O’Brien, V., de Jong, R., van Esch, J., Oldenhof, S., & de Puit, M. (2019). Non-marking collection of amino acids from fingerprints using hydrogels. In M. A. Alterman (Ed.), Amino acid analysis: methods and protocols (2 ed., pp. 429-438). (Methods in molecular biology (Clifton, N.J.); Vol. 2030). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9639-1_32
Published by  Kenniscentrum Techniek 26 July 2019

Publication date

Jul 2019


Vincent O’Brien
Robin de Jong
Jan van Esch
Sander Oldenhof
Marcel de Puit